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Congresso Brasileiro de Microbiologia 2023
 		Resumo: 353-1

353-1

PHYTASE, PROTEASE AND XYLANASE PRODUCTION BY Aspergillus niveus USING RICE STRAW AS CARBON SOURCE AND APPLICATION OF THE ENZYMES IN ANIMAL FEED

Autores:
Ana Lorena de Oliveira Simas (UFMS - Universidade Federal de Mato Grosso do Sul) ; Nathalia Nunes Glienke (UFMS - Universidade Federal de Mato Grosso do Sul) ; Nelciele Cavalieri de Alencar Guimarăes (UFMS - Universidade Federal de Mato Grosso do Sul) ; Rodrigo Mattos Silva Galeano (UFMS - Universidade Federal de Mato Grosso do Sul) ; Giovana Cristina Giannesi (UFMS - Universidade Federal de Mato Grosso do Sul)

Resumo:
Ingredients of vegetal origin contain antinutritional factors, such as phytic acid and non-starch polysaccharides that limit the utilization of nutrients by animals. Xylanases, proteases and phytases have been widely used with the objective of reducing the antinutritional properties of animal feed. The use of bioactives in animal nutrition can promote benefits associated with intestinal health, increasing the availability of nutrients to the animal and it can improve animal performance. Therefore, this study produced and analyzed the biochemical properties of phytases, proteases and xylanases from Aspergillus niveus using alternative carbon sources in semi-solid medium and evaluated the application of the enzymes in the hydrolysis of animal feed. Aspergillus niveus had the best production of phytase (rice straw), protease (crushed brown rice) and xylanase (wheat bran), with 1.93 U/mg, 0.75 ± 0.03 U/mg and 4.66 ± 1.38 U/mg, respectively. The A. niveus phytase (PhyAn) showed activity peak at pH and temperature of 4.5-5 and 45°C, respectively. The optimal temperature and pH for A. niveus protease (ProAn) were 50°C and pH 6, and for A. niveus xylanase (XylAn) 45°C and pH 5, respectively. In the in vitro feed tests, PhyAn and commercial phytase showed the best activities in wheat bran (2.21 ± 0.15 µmol/mL) and rice bran with 1.86 ± 0.04 µmol/mL, respectively. ProAn and the commercial protease showed better activity in rice bran (8.34 ± 0.76 µmol/mL) and alternative diet – pure fiber (5.57 ± 0.14 µmol/mL). The XylAn promoted the hydrolysis of all feeds in the different times tested, with superior results to the commercial xylanase. The 8-hour incubation of XylAn and the commercial enzyme in animal food showed best results in corn (38.63 ± 5.22 µmol/mL) and in the ADF (9.81 ± 1.75 µmol/mL) diet, respectively. The use of enzymes that resist the conditions of the gastrointestinal tract and with potential for action on animal feed are important to be applied in the agroindustry.

Palavras-chave:
 Aspergillus niveus, animal feed, phytase, protease, xylanase


Agęncia de fomento:
CNPq and CAPES